Myeloid Cell Derived IL1β Contributes to Pulmonary Hypertension in HFpEF.

ABSTRACT:

Background

Pulmonary hypertension (PH) in heart failure with preserved ejection fraction (HFpEF) is a common and highly morbid syndrome, but mechanisms driving PH-HFpEF are poorly understood. We sought to determine whether a well-accepted murine model of HFpEF also displays features of PH, and we sought to identify pathways that might drive early remodeling of the pulmonary vasculature in HFpEF.

Methods

Eight-week-old male and female C57BL/6J mice received either N^γ-nitro-L-arginine methyl ester and high-fat diet or control water and diet for 2, 5, and 12 weeks. The db/db mice were studied as a second model of HFpEF. Early pathways regulating PH were identified by bulk and single-cell RNA sequencing. Findings were confirmed by immunostain in lungs of mice or lung

SUBMITTER: Agrawal V 

PROVIDER: S-EPMC10655859 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature