Global Analysis of Post-Translational Side-Chain Arginylation Using Pan-Arginylation Antibodies.

ABSTRACT: Arginylation is a post-translational modification mediated by the arginyltransferase 1 (ATE1), which transfers the amino acid arginine to a protein or peptide substrate from a tRNA molecule. Initially, arginylation was thought to occur only on N-terminally exposed acidic residues, and its function was thought to be limited to targeting proteins for degradation. However, more recent data have shown that ATE1 can arginylate side chains of internal acidic residues in a protein without necessarily affecting metabolic stability. This greatly expands the potential targets and functions of arginylation, but tools for studying this process have remained limited. Here, we report the first global screen specifically for side-chain arginylation. We generate and validate "pan-arginylation" antibodies,

SUBMITTER: MacTaggart B 

PROVIDER: S-EPMC10656225 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature