Mutant p53^R211* ameliorates inflammatory arthritis in AIA rats via inhibition of TBK1-IRF3 innate immune response.

ABSTRACT:

Background

Rheumatoid arthritis (RA) is an autoimmune inflammation disease characterized by imbalance of immune homeostasis. p53 mutants are commonly described as the guardian of cancer cells by conferring them drug-resistance and immune evasion. Importantly, p53 mutations have also been identified in RA patients, and this prompts the investigation of its role in RA pathogenesis.

Methods

The cytotoxicity of disease-modifying anti-rheumatic drugs (DMARDs) against p53 ^{wild-type (WT)/mutant}-transfected RA fibroblast-like synoviocytes (RAFLSs) was evaluated by MTT assay. Adeno-associated virus (AAV) was employed to establish p53 ^WT/R211* adjuvant-induced arthritis (AIA) rat model. The arthritic condition of rats was assessed by various parameters such as mi

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC10656327 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature