Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules.

ABSTRACT: Controlling gene expression in response to specific molecules is an essential technique for regulating cellular functions. However, current platforms with transcription and translation regulators have a limited number of detectable molecules to induce gene expression. Here to address these issues, we present a Target-dependent RNA polymerase (TdRNAP) that can induce RNA transcription in response to the intracellular target specifically recognized by single antibody. By substituting the fused antibody, we demonstrate that TdRNAPs respond to a wide variety of molecules, including peptides, proteins, RNA, and small molecules, and produce desired transcripts in human cells. Furthermore, we show that multiple TdRNAPs can construct orthogonal and multilayer genetic circuits. Finally, we apply Td

SUBMITTER: Komatsu S 

PROVIDER: S-EPMC10656481 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature