TOF_IMS mass spectrometry-based immunopeptidomics refines tumor antigen identification.

ABSTRACT: T cell recognition of human leukocyte antigen (HLA)-presented tumor-associated peptides is central for cancer immune surveillance. Mass spectrometry (MS)-based immunopeptidomics represents the only unbiased method for the direct identification and characterization of naturally presented tumor-associated peptides, a key prerequisite for the development of T cell-based immunotherapies. This study reports on the implementation of ion mobility separation-based time-of-flight (TOF_IMS) MS for next-generation immunopeptidomics, enabling high-speed and sensitive detection of HLA-presented peptides. Applying TOF_IMS-based immunopeptidomics, a novel extensive benign_TOFIMS dataset was generated from 94 primary benign samples of solid tissue and hematological origin, wh

SUBMITTER: Hoenisch Gravel N 

PROVIDER: S-EPMC10656517 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature