The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli.

ABSTRACT: Nucleoid associated proteins (NAPs) maintain the architecture of bacterial chromosomes and regulate gene expression. Thus, their role as transcription factors may involve three-dimensional chromosome re-organisation. While this model is supported by in vitro studies, direct in vivo evidence is lacking. Here, we use RT-qPCR and 3C-qPCR to study the transcriptional and architectural profiles of the H-NS (histone-like nucleoid structuring protein)-regulated, osmoresponsive proVWX operon of Escherichia coli at different osmolarities and provide in vivo evidence for transcription regulation by NAP-mediated chromosome re-modelling in bacteria. By consolidating our in vivo investigations with earlier in vitro and in silico studies that provide mechanistic details of how H-NS re-models DNA in resp

SUBMITTER: Rashid FM 

PROVIDER: S-EPMC10656529 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature