Integrative transcriptome analysis reveals alternative polyadenylation potentially contributes to GCRV early infection.

ABSTRACT:

Introduction

Grass carp reovirus (GCRV), a member of the Aquareovirus genus in the Reoviridae family, is considered to be the most pathogenic aquareovirus. Productive viral infection requires extensive interactions between viruses and host cells. However, the molecular mechanisms underlying GCRV early infection remains elusive.

Methods

In this study we performed transcriptome and DNA methylome analyses with Ctenopharyngodon idellus kidney (CIK) cells infected with GCRV at 0, 4, and 8 h post infection (hpi), respectively.

Results

We found that at early infection stage the differentially expressed genes related to defense response and immune response in CIK cells are activated. Although DNA methylation pattern of CIK cells 8 hpi is similar to mock-i

SUBMITTER: Tan S 

PROVIDER: S-EPMC10656684 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature