Dataset Information

A Wohlfahrtiimonas chitiniclastica with a novel type of bla_VEB-1-carrying plasmid isolated from a zebra in China.

ABSTRACT:

Background

Wohlfahrtiimonas chitiniclastica is an emerging fly-borne zoonotic pathogen, which causes infections in immunocompromised patients and some animals. Herein, we reported a W. chitiniclastica BM-Y from a dead zebra in China.

Methods

The complete genome sequencing of BM-Y showed that this isolate carried one chromosome and one novel type of bla_VEB-1-carrying plasmid. Detailed genetic dissection was applied to this plasmid to display the genetic environment of bla_VEB-1.

Results

Three novel insertion sequence (IS) elements, namely ISWoch1, ISWoch2, and ISWoch3, were found in this plasmid. aadB, aacA1, and gcuG were located downstream of bla_VEB-1, composing a gene cassette array bla_VEB-1-aadB-aacA1-gcuG bracketed by an intact ISWoch1 and a truncated one, which was named the bla_VEB-1 region. The 5'-RACE experiments revealed that the transcription start site of the bla_VEB-1 region was located in the intact ISWoch1 and this IS provided a strong promoter for the bla_VEB-1 region.

Conclusion

The spread of the bla_VEB-1-carrying plasmid might enhance the ability of W. chitiniclastica to survive under drug selection pressure and aggravate the difficulty in treating infections caused by bla_VEB-1-carrying W. chitiniclastica. To the best of our knowledge, this is the first report of the genetic characterization of a novel bla_VEB-1-carrying plasmid with new ISs from W. chitiniclastica.

SUBMITTER: Guan J

PROVIDER: S-EPMC10656743 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

A <i>Wohlfahrtiimonas chitiniclastica</i> with a novel type of <i>bla</i><sub>VEB-1</sub>-carrying plasmid isolated from a zebra in China.

Guan Jiayao J Zhou Wei W Guo Jingyi J Zheng Lin L Lu Gejin G Hua Fuyou F Liu Mingwei M Ji Xue X Sun Yang Y Zhu Lingwei L Guo Xuejun X

Frontiers in microbiology 20231102

<h4>Background</h4><i>Wohlfahrtiimonas chitiniclastica</i> is an emerging fly-borne zoonotic pathogen, which causes infections in immunocompromised patients and some animals. Herein, we reported a <i>W. chitiniclastica</i> BM-Y from a dead zebra in China.<h4>Methods</h4>The complete genome sequencing of BM-Y showed that this isolate carried one chromosome and one novel type of <i>bla</i><sub>VEB-1</sub>-carrying plasmid. Detailed genetic dissection was applied to this plasmid to display the gene ...[more]

PMID: 38029080

Similar Datasets

Project description:While conducting genomic surveillance of carbapenemase-producing Enterobacteriaceae (CPE) from patient colonisation and clinical infections at Birmingham's Queen Elizabeth Hospital (QE), we identified an N-type plasmid lineage, pQEB1, carrying several antibiotic resistance genes, including the carbapenemase gene bla KPC-2. The pQEB1 lineage is concerning due to its conferral of multidrug resistance, its host range and apparent transmissibility, and its potential for acquiring further resistance genes. Representatives of pQEB1 were found in three sequence types (STs) of Citrobacter freundii, two STs of Enterobacter cloacae, and three species of Klebsiella. Hosts of pQEB1 were isolated from 11 different patients who stayed in various wards throughout the hospital complex over a 13 month period from January 2023 to February 2024. At present, the only representatives of the pQEB1 lineage in GenBank were carried by an Enterobacter hormaechei isolated from a blood sample at the QE in 2016 and a Klebsiella pneumoniae isolated from a urine sample at University Hospitals Coventry and Warwickshire (UHCW) in May 2023. The UHCW patient had been treated at the QE. Long-read whole-genome sequencing was performed on Oxford Nanopore R10.4.1 flow cells, facilitating comparison of complete plasmid sequences. We identified structural variants of pQEB1 and defined the molecular events responsible for them. These have included IS26-mediated inversions and acquisitions of multiple insertion sequences and transposons, including carriers of mercury or arsenic resistance genes. We found that a particular inversion variant of pQEB1 was strongly associated with the QE Liver speciality after appearing in November 2023, but was found in different specialities and wards in January/February 2024. That variant has so far been seen in five different bacterial hosts from six patients, consistent with recent and ongoing inter-host and inter-patient transmission of pQEB1 in this hospital setting.

Dataset Information

A Wohlfahrtiimonas chitiniclastica with a novel type of bla_VEB-1-carrying plasmid isolated from a zebra in China.

Background

Methods

Results

Conclusion

Publications

A <i>Wohlfahrtiimonas chitiniclastica</i> with a novel type of <i>bla</i><sub>VEB-1</sub>-carrying plasmid isolated from a zebra in China.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

A Wohlfahrtiimonas chitiniclastica with a novel type of blaVEB-1-carrying plasmid isolated from a zebra in China.

Background

Methods

Results

Conclusion

Publications

A <i>Wohlfahrtiimonas chitiniclastica</i> with a novel type of <i>bla</i><sub>VEB-1</sub>-carrying plasmid isolated from a zebra in China.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

A Wohlfahrtiimonas chitiniclastica with a novel type of bla_VEB-1-carrying plasmid isolated from a zebra in China.