In-house ELISA protocols for capsid p24 detection of diverse HIV isolates.

ABSTRACT:

Background

The capsid p24 (CA-p24) antigen is a component of the viral capsid of human immunodeficiency virus (HIV) that has been commonly used for clinical diagnosis and monitoring of HIV infections in Enzyme-linked Immunosorbent Assays (ELISAs). Commercial CA-p24 ELISAs are widely used in research settings, but these kits are costly and have limited breadth for detecting diverse HIV isolates.

Methods

Commercial CA-p24 antibodies were used as capture and detection antibodies. Specific CA-p24 ELISAs were established with these antibodies and tested for the detection of HIV-1 isolates with the aim of developing in-house protocols to recognize HIV-1 infections in vitro for research purposes.

Results

Here we present four protocols for in-house ELISAs to detect HIV CA-p24

SUBMITTER: Molina MA 

PROVIDER: S-EPMC10656996 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature