Migration speed of captured breast cancer subpopulations correlates with metastatic fitness.

ABSTRACT: The genetic alterations contributing to migration proficiency, a phenotypic hallmark of metastatic cells required for colonizing distant organs, remain poorly defined. Here, we used single-cell magneto-optical capture (scMOCa) to isolate fast cells from heterogeneous human breast cancer cell populations, based on their migratory ability alone. We show that captured fast cell subpopulations retain higher migration speed and focal adhesion dynamics over many generations as a result of a motility-related transcriptomic profile. Upregulated genes in isolated fast cells encoded integrin subunits, proto-cadherins and numerous other genes associated with cell migration. Dysregulation of several of these genes correlates with poor survival outcomes in people with breast cancer, and primary tumors

SUBMITTER: Desjardins-Lecavalier N 

PROVIDER: S-EPMC10657211 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature