MvaT binds to the P_exsC promoter to repress the type III secretion system in Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic human pathogen capable of causing a variety of acute and chronic infections. Its type III secretion system (T3SS) plays a critical role in pathogenesis during acute infection. ExsA is a master regulator that activates the expression of all T3SS genes. Transcription of exsA is driven by two distinct promoters, its own promoter P_exsA and its operon promoter P_exsC. Here, in combination with a DNA pull-down assay and mass spectrometric analysis, we found that a histone-like nucleoid-structuring (H-NS) family protein MvaT can bind to the P_exsC promoter. Using EMSA and reporter assays, we further found that MvaT directly binds to the P_exsC promoter to repress the expre

SUBMITTER: Yin L 

PROVIDER: S-EPMC10657842 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature