Bac to Bac System Efficiency for Preparing HPV Type 16 Virus-Like Particle Vaccine.

ABSTRACT: Today, the human papillomavirus (HPV) L1 protein is the main target in the construction of prophylactic HPV vaccines. The production of virus-like particles (VLPs) that closely resemble the natural structure of the HPV16 virus and induce high levels of virus-neutralizing antibodies in animals and humans is facilitated by the expression of HPV16-L1 protein in eukaryotic cells. The Bac-to-Bac system has been previously used to produce high levels of recombinant proteins. In this study, we utilized this expression system to generate HPV16-L1 VLPs in Spodoptra frugipedra (Sf9) insect cells. The wild-type L1 gene of papillomavirus type 16 was selected from Gene Bank and placed in bacmid structure after codon optimization using pFast Bac vector. The recombinant baculovirus containing HPV-

SUBMITTER: Razavi-Nikoo H 

PROVIDER: S-EPMC10657962 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature