Intestinal microbiota dysbiosis and liver metabolomic changes during brain death.

ABSTRACT:

Background

Whether a causative link exists between brain death (BD) and intestinal microbiota dysbiosis is unclear, and the distortion in liver metabolism associated with BD requires further exploration.

Methods

A rat model of BD was constructed and sustained for 9 h (BD group, n=6). The sham group (n=6) underwent the same procedures, but the catheter was inserted into the epidural space without ballooning. Intestinal contents and portal vein plasma were collected for microbiota sequencing and microbial metabolite detection. Liver tissue was resected to investigate metabolic alterations, and the results were compared with those of a sham group.

Results

α-diversity indexes showed that BD did not alter bacterial diversity. Microbiota dysbiosis occurred aft

SUBMITTER: Tao R 

PROVIDER: S-EPMC10658038 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature