An efficient evaluation system for factors affecting the genome editing efficiency in mouse.

ABSTRACT: Genome editing technology is widely used in the field of laboratory animal science for the production of genetic disease models and the analysis of gene function. One of the major technical problems in genome editing is the low efficiency of precise knock-in by homologous recombination compared to simple knockout via non-homologous end joining. Many studies have focused on this issue, and various solutions have been proposed; however, they have yet to be fully resolved. In this study, we established a system that can easily determine the genotype at the mouse (Mus musculus) Tyr gene locus for genome editing both in vitro and in vivo. In this genome editing system, by designing the Cas9 cleavage site and donor template, wild-type, knockout, and knock-in genotypes can be distinguished by res

SUBMITTER: Sakai Y 

PROVIDER: S-EPMC10658088 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature