The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency.

ABSTRACT:

Introduction

Meiotic recombination is one of the most important processes of evolution and adaptation to environmental conditions. Even though there is substantial knowledge about proteins involved in the process, targeting specific DNA loci by the recombination machinery is not well understood.

Objectives

This study aims to investigate a wheat recombination hotspot (H1) in comparison with a "regular" recombination site (Rec7) on the sequence and epigenetic level in conditions with functional and non-functional Ph1 locus.

Methods

The DNA sequence, methylation pattern, and recombination frequency were analyzed for the H1 and Rec7 in three mapping populations derived by crossing introgressive wheat line 8.1 with cv. Chinese Spring (with Ph1 and ph1 alleles) and cv. Täht

SUBMITTER: Majka M 

PROVIDER: S-EPMC10658417 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature