Histological assessment of nanostructured fibrin-agarose skin substitutes grafted in burnt patients. A time-course study.

ABSTRACT: A previously developed fibrin-agarose skin model-UGRSKIN-showed promising clinical results in severely burnt patients. To determine the histological parameters associated to the biocompatibility and therapeutic effects of this model, we carried out a comprehensive structural and ultrastructural study of UGRSKIN grafted in severely burnt patients after 3 months of follow-up. The grafted epidermis was analogue to native human skin from day 30th onward, revealing well-structured strata with well-differentiated keratinocytes expressing CK5, CK8, CK10, claudin, plakoglobin, filaggrin, and involucrin in a similar way to controls, suggesting that the epidermis was able to mature and differentiate very early. Melanocytes and Langerhans cells were found from day 30th onward, together with a basemen

SUBMITTER: Martin-Piedra MA 

PROVIDER: S-EPMC10658487 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature