Organ-on-a-chip model of vascularized human bone marrow niches.

ABSTRACT: Bone marrow niches (endosteal and perivascular) play important roles in both normal bone marrow function and pathological processes such as cancer cell dormancy. Unraveling the mechanisms underlying these events in humans has been severely limited by models that cannot dissect dynamic events at the niche level. Utilizing microfluidic and stem cell technologies, we present a 3D in vitro model of human bone marrow that contains both the perivascular and endosteal niches, complete with dynamic, perfusable vascular networks. We demonstrate that our model can replicate in vivo bone marrow function, including maintenance and differentiation of CD34⁺ hematopoietic stem/progenitor cells, egress of neutrophils (CD66b⁺), and niche-specific responses to doxorubicin and granulocy

SUBMITTER: Glaser DE 

PROVIDER: S-EPMC10658812 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature