High-resolution dynamic mapping of the C. elegans intestinal brush border.

ABSTRACT: The intestinal brush border is made of an array of microvilli that increases the membrane surface area for nutrient processing, absorption and host defense. Studies on mammalian cultured epithelial cells have uncovered some of the molecular players and physical constraints required to establish this apical specialized membrane. However, the building and maintenance of a brush border in vivo has not yet been investigated in detail. Here, we combined super-resolution imaging, transmission electron microscopy and genome editing in the developing nematode Caenorhabditis elegans to build a high-resolution and dynamic localization map of known and new brush border markers. Notably, we show that microvilli components are dynamically enriched at the apical membrane during microvilli outgrowth and

SUBMITTER: Bidaud-Meynard A 

PROVIDER: S-EPMC10659032 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature