Lactate: an alternative pathway for the immunosuppressive properties of mesenchymal stem/stromal cells.

ABSTRACT:

Background

The metabolic reprogramming of mesenchymal stem/stromal cells (MSC) favoring glycolysis has recently emerged as a new approach to improve their immunotherapeutic abilities. This strategy is associated with greater lactate release, and interestingly, recent studies have proposed lactate as a functional suppressive molecule, changing the old paradigm of lactate as a waste product. Therefore, we evaluated the role of lactate as an alternative mediator of MSC immunosuppressive properties and its contribution to the enhanced immunoregulatory activity of glycolytic MSCs.

Materials and methods

Murine CD4⁺ T cells from C57BL/6 male mice were differentiated into proinflammatory Th1 or Th17 cells and cultured with either L-lactate, MSCs pretreated or not with the

SUBMITTER: Pradenas C 

PROVIDER: S-EPMC10659074 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature