Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings.

ABSTRACT: The road to malaria elimination for low- and middle-income countries is paved with obstacles, including the complexity and high costs of advanced molecular methods for genomic analysis. The usefulness of PCR-RFLP as less complex and affordable molecular surveillance tool in low-endemic malaria regions was assessed in a cross-sectional study conducted in Suriname, currently striving for malaria elimination, but plagued by recent P. vivax outbreaks. Molecular analysis of two highly polymorphic genes Pvmsp-1 F2 and Pvmsp-3α was performed for 49 samples, collected during October 2019 through September 2021 from four different regions with varying malaria transmission risks. RFLP-profiling revealed that outbreak samples from three indigenous villages, almost exclusively, harbored a single clona

SUBMITTER: Labadie-Bracho MY 

PROVIDER: S-EPMC10659184 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature