Self Fourier shell correlation: properties and application to cryo-ET.

ABSTRACT: The Fourier shell correlation (FSC) is a measure of the similarity between two signals computed over corresponding shells in the frequency domain and has broad applications in microscopy. In structural biology, the FSC is ubiquitous in methods for validation, resolution determination, and signal enhancement. Computing the FSC usually requires two independent measurements of the same underlying signal, which can be limiting for some applications. Here, we analyze and extend on an approach proposed by Koho et al. [1] to estimate the FSC from a single measurement. In particular, we derive the necessary conditions required to estimate the FSC from downsampled versions of a single noisy measurement. These conditions reveal additional corrections which we implement to increase the applicability

SUBMITTER: Verbeke EJ 

PROVIDER: S-EPMC10659293 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature