Degron-controlled protein degradation in Escherichia coli: New Approaches and Parameters.

ABSTRACT: Protein degron tags have proven uniquely useful for characterization of gene function. Degrons mediate quick depletion, usually within minutes, of a protein of interest - allowing researchers to characterize cellular responses to the loss of function. To develop a general purpose degron tool in E. coli, we sought to build upon a previously characterized system of SspB-dependent inducible protein degradation. For this, we created a family of expression vectors containing a destabilized allele of SspB, capable of a rapid and nearly perfect "off-to-on" induction response. Using this system, we demonstrated control over several enzymes of DNA metabolism, but also found with other substates apparent limitations of a SspB-dependent system. Several degron target proteins were degraded too

SUBMITTER: Cronan GE 

PROVIDER: S-EPMC10659297 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature