Aberrant hippocampal Ca²⁺ micro-waves following synapsin-dependent adeno-associated viral expression of Ca²⁺ indicators.

ABSTRACT: Genetically encoded calcium indicators (GECIs) such as GCaMP are invaluable tools in neuroscience to monitor neuronal activity using optical imaging. The viral transduction of GECIs is commonly used to target expression to specific brain regions, can be conveniently used with any mouse strain of interest without the need for prior crossing with a GECI mouse line and avoids potential hazards due to the chronic expression of GECIs during development. A key requirement for monitoring neuronal activity with an indicator is that the indicator itself minimally affects activity. Here, using common adeno-associated viral (AAV) transduction procedures, we describe spatially confined aberrant Ca²⁺ micro-waves slowly travelling through the hippocampus following expression of GCaMP6, GCaMP7

SUBMITTER: Masala N 

PROVIDER: S-EPMC10659308 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature