Metabolic Regulation of Single Synaptic Vesicle Exo- and Endocytosis in Hippocampal Synapses.

ABSTRACT: Glucose has long been considered a primary source of energy for synaptic function. However, it remains unclear under what conditions alternative fuels, such as lactate/pyruvate, contribute to powering synaptic transmission. By detecting individual release events in cultured hippocampal synapses, we found that mitochondrial ATP production from oxidation of lactate/pyruvate regulates basal vesicle release probability and release location within the active zone (AZ) evoked by single action potentials (APs). Mitochondrial inhibition shifted vesicle release closer to the AZ center, suggesting that the energetic barrier for vesicle release is lower in the AZ center that the periphery. Mitochondrial inhibition also altered the efficiency of single AP evoked vesicle retrieval by increasing occurre

SUBMITTER: Myeong J 

PROVIDER: S-EPMC10659320 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature