Transcriptomic changes during the replicative senescence of human articular chondrocytes.

ABSTRACT: Osteoarthritis (OA) is a degenerative joint disease and a leading cause of disability worldwide. Aging is a major risk factor for OA, but the specific mechanisms underlying this connection remain unclear. Although chondrocytes rarely divide in adult articular cartilage, they undergo replicative senescence in vitro which provides an opportunity to study changes related to aging under controlled laboratory conditions. In this pilot study, we performed bulk RNA sequencing on early- and late-passage human articular chondrocytes to identify transcriptomic changes associated with cellular aging. Chondrocytes were isolated from the articular cartilage of three donors, two with OA (age 70-80 years) and one with healthy cartilage (age 26 years). Chondrocytes were serially passaged until repl

SUBMITTER: Atasoy-Zeybek A 

PROVIDER: S-EPMC10659330 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature