ADP release can explain spatially-dependent kinesin binding times.

ABSTRACT: The self-organization of cells relies on the profound complexity of protein-protein interactions. Challenges in directly observing these events have hindered progress toward understanding their diverse behaviors. One notable example is the interaction between molecular motors and cytoskeletal systems that combine to perform a variety of cellular functions. In this work, we leverage theory and experiments to identify and quantify the rate-limiting mechanism of the initial association between a cargo-bound kinesin motor and a microtubule track. Recent advances in optical tweezers provide binding times for several lengths of kinesin motors trapped at varying distances from a microtubule, empowering the investigation of competing models. We first explore a diffusion-limited model of binding. T

SUBMITTER: Nguyen T 

PROVIDER: S-EPMC10659338 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature