Shaping human gut community assembly and butyrate production by controlling the arginine dihydrolase pathway.

ABSTRACT: The arginine dihydrolase pathway (arc operon) present in a subset of diverse human gut species enables arginine catabolism. This specialized metabolic pathway can alter environmental pH and nitrogen availability, which in turn could shape gut microbiota inter-species interactions. By exploiting synthetic control of gene expression, we investigated the role of the arc operon in probiotic Escherichia coli Nissle 1917 on human gut community assembly and health-relevant metabolite profiles in vitro and in the murine gut. By stabilizing environmental pH, the arc operon reduced variability in community composition across different initial pH perturbations. The abundance of butyrate producing bacteria were altered in response to arc operon activity and bu

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10659395 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature