Comparing the effects of chemical Ca²⁺ dyes and R-GECO on contractility and Ca²⁺ transients in adult and human iPSC cardiomyocytes.

ABSTRACT: We compared commonly used BAPTA-derived chemical Ca²⁺ dyes (fura2, Fluo-4, and Rhod-2) with a newer genetically encoded indicator (R-GECO) in single cell models of the heart. We assessed their performance and effects on cardiomyocyte contractility, determining fluorescent signal-to-noise ratios and sarcomere shortening in primary ventricular myocytes from adult mouse and guinea pig, and in human iPSC-derived cardiomyocytes. Chemical Ca²⁺ dyes displayed dose-dependent contractile impairment in all cell types, and we observed a negative correlation between contraction and fluorescence signal-to-noise ratio, particularly for fura2 and Fluo-4. R-GECO had no effect on sarcomere shortening. BAPTA-based dyes, but not R-GECO, inhibited in vitro acto-myosin ATPase activity. Th

SUBMITTER: Robinson P 

PROVIDER: S-EPMC10659987 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature