Assay validation of saliva glucocorticoids in Columbia spotted frogs and effects of handling and marking.

ABSTRACT: Non-invasive methods are important to the field of conservation physiology to reduce negative effects on organisms being studied. Glucocorticoid (GC) hormones are often used to assess health of individuals, but collection methods can be invasive. Many amphibians are imperiled worldwide, and saliva is a non- or semi-invasive matrix to measure GCs that has been partially validated for only four amphibian species. Validation ensures that assays are reliable and can detect changes in saliva corticosterone (sCORT) after exposure to stressors, but it is also necessary to ensure sCORT concentrations are correlated with plasma concentrations. To help validate the use of saliva in assessing CORT responses in amphibians, we captured uniquely marked Columbia spotted frogs (Rana luteiventris) o

SUBMITTER: Tornabene BJ 

PROVIDER: S-EPMC10660366 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature