Super-resolution microscopy reveals the nanoscale cluster architecture of the DEK protein cancer biomarker.

ABSTRACT: DEK protein, a key chromatin regulator, is strongly overexpressed in various forms of cancer. While conventional microscopy revealed DEK as uniformly distributed within the cell nucleus, advanced super-resolution techniques uncovered cluster-like structures. However, a comprehensive understanding of DEK's cellular distribution and its implications in cancer and cell growth remained elusive. To bridge this gap, we employed single-molecule localization microscopy (SMLM) to dissect DEK's nanoscale organization in both normal-like and aggressive breast cancer cell lines. Our investigation included characteristics such as localizations per cluster, cluster areas, and intra-cluster localization densities (ICLDs). We elucidated how cluster features align with different breast cell types and how c

SUBMITTER: Pierzynska-Mach A 

PROVIDER: S-EPMC10660485 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature