Investigating and Modeling the Factors That Affect Genetic Circuit Performance.

ABSTRACT: Over the past 2 decades, synthetic biology has yielded ever more complex genetic circuits that are able to perform sophisticated functions in response to specific signals. Yet, genetic circuits are not immediately transferable to an outside-the-lab setting where their performance is highly compromised. We propose introducing a broader test step to the design-build-test-learn workflow to include factors that might contribute to unexpected genetic circuit performance. As a proof of concept, we have designed and evaluated a genetic circuit in various temperatures, inducer concentrations, nonsterilized soil exposure, and bacterial growth stages. We determined that the circuit's performance is dramatically altered when these factors differ from the optimal lab conditions. We observed significan

SUBMITTER: Zilberzwige-Tal S 

PROVIDER: S-EPMC10661042 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature