Module-Based Polyketide Synthase Engineering for de Novo Polyketide Biosynthesis.

ABSTRACT: Polyketide retrobiosynthesis, where the biosynthetic pathway of a given polyketide can be reversibly engineered due to the colinearity of the polyketide synthase (PKS) structure and function, has the potential to produce millions of organic molecules. Mixing and matching modules from natural PKSs is one of the routes to produce many of these molecules. Evolutionary analysis of PKSs suggests that traditionally used module boundaries may not lead to the most productive hybrid PKSs and that new boundaries around and within the ketosynthase domain may be more active when constructing hybrid PKSs. As this is still a nascent area of research, the generality of these design principles based on existing engineering efforts remains inconclusive. Recent advances in structural modeling and synthetic

SUBMITTER: Nava AA 

PROVIDER: S-EPMC10661043 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature