Validating a Promoter Library for Application in Plasmid-Based Diatom Genetic Engineering.

ABSTRACT: While diatoms are promising synthetic biology platforms, there currently exists a limited number of validated genetic regulatory parts available for genetic engineering. The standard method for diatom transformation, nonspecific introduction of DNA into chromosomes via biolistic particle bombardment, is low throughput and suffers from clonal variability and epigenetic effects. Recent developments in diatom engineering have demonstrated that autonomously replicating episomal plasmids serve as stable expression platforms for diverse gene expression technologies. These plasmids are delivered via bacterial conjugation and, when combined with modular DNA assembly technologies, provide a flexibility and speed not possible with biolistic-mediated strain generation. In order to expand the current

SUBMITTER: Garza EA 

PROVIDER: S-EPMC10661051 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature