Development of [⁸⁹Zr]Zr-hCD103.Fab01A and [⁶⁸Ga]Ga-hCD103.Fab01A for PET imaging to noninvasively assess cancer reactive T cell infiltration: Fab-based CD103 immunoPET.

ABSTRACT:

Background

CD103 is an integrin specifically expressed on the surface of cancer-reactive T cells. The number of CD103+ T cells significantly increases during successful immunotherapy and might therefore be an attractive biomarker for noninvasive PET imaging of immunotherapy response. Since the long half-life of antibodies preclude repeat imaging of CD103+ T cell dynamics early in therapy, we therefore here explored PET imaging with CD103 Fab fragments radiolabeled with a longer (⁸⁹Zr) and shorter-lived radionuclide (⁶⁸Ga).

Methods

Antihuman CD103 Fab fragment Fab01A was radiolabeled with ⁸⁹Zr or ⁶⁸Ga, generating [⁸⁹Zr]Zr-hCD103.Fab01A and [⁶⁸Ga]Ga-hCD103.Fab01A, respectively. In vivo evaluation of these tracer

SUBMITTER: Fan X 

PROVIDER: S-EPMC10661679 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature