Ethanol deprivation and central 5-HT deficiency differentially affect the mRNA editing of the 5-HT_2C receptor in the mouse brain.

ABSTRACT:

Background

Serotonin (5-HT) 5-HT_2C receptor mRNA editing (at five sites, A-E), implicated in neuropsychiatric disorders, including clinical depression, remains unexplored during alcohol abstinence-often accompanied by depressive symptoms.

Methods

We used deep sequencing to investigate 5-HT_2C receptor editing in mice during early ethanol deprivation following prolonged alcohol exposure and mice lacking tryptophan hydroxylase (TPH)2, a key enzyme in central 5-HT production. We also examined Tph2 expression in ethanol-deprived animals using quantitative real-time PCR (qPCR).

Results

Cessation from chronic 10% ethanol exposure in a two-bottle choice paradigm enhanced immobility time and decreased latency in the forced swim test (FST), indicating a de

SUBMITTER: Zaniewska M 

PROVIDER: S-EPMC10661786 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature