Protein engineering of multi-enzyme virus-like particle nanoreactors for enhanced chiral alcohol synthesis.

ABSTRACT: In the past decade, virus-like particles (VLPs) that can encapsulate single or multiple enzymes have been studied extensively as typical nanoreactors for biocatalysis in vitro, yet their catalytic efficiencies are usually inadequate for real applications. These biocatalytic nanoreactors should be engineered like their free-enzyme counterparts to improve their catalytic performance for potential applications. Herein we engineer biocatalytic VLPs for the enhanced synthesis of chiral alcohols. Different methods including directed evolution were applied to the entire bacteriophage P22 VLPs (except the coat protein), which encapsulated a carbonyl reductase from Scheffersomyces stipitis (SsCR) and a glucose dehydrogenase from Bacillus megaterium (BmGDH) in thei

SUBMITTER: Feng T 

PROVIDER: S-EPMC10662152 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature