A core-genome multilocus sequence typing scheme for the detection of genetically related Streptococcus pyogenes clusters.

ABSTRACT: The recently observed increase in invasive Streptococcus pyogenes infections causes concern in Europe. However, conventional molecular typing methods lack discriminatory power to aid investigations of outbreaks caused by S. pyogenes. Therefore, there is an urgent need for high-resolution molecular typing methods to assess genetic relatedness between S. pyogenes isolates. In the current study, we aimed to develop a novel high-resolution core-genome multilocus sequence typing (cgMLST) scheme for S. pyogenes and compared its discriminatory power to conventional molecular typing methods. The cgMLST scheme was designed with the commercial Ridom SeqSphere+ software package. To define a cluster threshold, the scheme was evaluated using publicly available data from nine

SUBMITTER: Toorop MMA 

PROVIDER: S-EPMC10662357 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature