Probing RNA-Small Molecule Interactions Using Biophysical and Computational Approaches.

ABSTRACT: Interest in small molecules that target RNA is flourishing, and the expectation set on them to treat diseases with unmet medical needs is high. However, several challenges remain, including difficulties in selecting suitable tools and establishing workflows for their discovery. In this context, we optimized experimental and computational approaches that were previously employed for the protein targets. Here, we demonstrate that a fluorescence-based assay can be effectively used to screen small molecule libraries for their ability to bind and stabilize an RNA stem-loop. Our screen identified several fluoroquinolones that bind to the target stem-loop. We further probed their interactions with the target using biolayer interferometry, isothermal titration calorimetry (ITC), and nuclear magnet

SUBMITTER: Shino A 

PROVIDER: S-EPMC10662358 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature