Defining the minimal components of the influenza A virus replication machinery via an in vitro reconstitution system.

ABSTRACT: During influenza A virus infection, the viral RNA polymerase transcribes the viral negative-sense segmented RNA genome and replicates it in a two-step process via complementary RNA within viral ribonucleoprotein (vRNP) complexes. While numerous viral and host factors involved in vRNP functions have been identified, dissecting the roles of individual factors remains challenging due to the complex cellular environment in which vRNP activity has been studied. To overcome this challenge, we reconstituted viral transcription and a full cycle of replication in a test tube using vRNPs isolated from virions and recombinant factors essential for these processes. This novel system uncovers the minimal components required for influenza virus replication and also reveals new roles of regulatory factor

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC10662765 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature