Transcriptome-wide profiling of acute stress induced changes in ribosome occupancy level using external standards.

ABSTRACT: Acute cellular stress is known to induce a global reduction in mRNA translation through suppression of cap dependent translation. Selective translation in response to acute stress has been shown to play important roles in regulating the stress response. However, accurately profiling translational changes transcriptome-wide in response to acute cellular stress has been challenging. Commonly used data normalization methods operate on the assumption that any systematic shifts are experimental artifacts. Consequently, if applied to profiling acute cellular stress-induced mRNA translation changes, these methods are expected to produce biased estimates. To address this issue, we designed, produced, and evaluated a panel of 16 oligomers to serve as external standards for ribosome profiling studie

SUBMITTER: Shieh AW 

PROVIDER: S-EPMC10662766 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature