Identification of target hub genes and construction of a novel miRNA regulatory network in autism spectrum disorder by integrated analysis.

ABSTRACT: The incidence of autism spectrum disorder (ASD) is increasing year by year in children. The aim of the study was to find possible biomarkers for ASD diagnosis as well as examine MicroRNA (miRNA) signatures and crucial pathways. We conducted a two-stage study to explore potential target genes and functional miRNAs. Peripheral blood samples of children with ASD were enrolled and performed RNA sequencing analysis. The overlapped candidate genes were further screened in combination with differentially expressed genes (DEGs) of GSE77103 datasets. STRING established a protein-protein interaction network comprising DEGs. The hub genes were filtered out using the CytoHubba. Then, we set up a miRNA-mRNA regulatory network. Correlational analyses between hub genes and immune cells associated with AS

SUBMITTER: Zhu J 

PROVIDER: S-EPMC10662836 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature