Bayesian Inference Elucidates the Catalytic Competency of the SARS-CoV-2 Main Protease 3CL^pro.

ABSTRACT: The main protease of SARS-CoV-2, 3CL^pro, is a dimeric enzyme that is indispensable to viral replication and presents an attractive opportunity for therapeutic intervention. Previous reports regarding the key properties of 3CL^pro and its highly similar SARS-CoV homologue conflict dramatically. Values of the dimeric K_d and enzymic k_cat/K_M differ by 10⁶- and 10³-fold, respectively. Establishing a confident benchmark of the intrinsic capabilities of this enzyme is essential for combating the current pandemic as well as potential future outbreaks. Here, we use enzymatic methods to characterize the dimerization and catalytic efficiency of the authentic protease from SARS-CoV-2. Specifically, we use

SUBMITTER: Wralstad EC 

PROVIDER: S-EPMC10662973 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature