Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor.

ABSTRACT: There is great interest in developing boronolectins that are synthetic lectin mimics containing a boronic acid functional group for reversible recognition of diol-containing molecules, such as glycans and ribonucleotides. However, it remains a significant challenge to gain specificity. Here, we present a genetically encoded boronolectin which is a hybrid protein consisting of a noncanonical amino acid (ncAA) p-boronophenylalanine (pBoF), natural-lectin-derived peptide sequences, and a circularly permuted red fluorescent protein (cpRFP). The genetic encodability permitted a straightforward protein engineering process to derive a red fluorescent biosensor that can specifically bind uridine diphosphate N-acetylglucosamine (UDP-GlcNAc), an important nucleotide sugar involv

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10663054 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature