Spatiotemporal distribution of reactive oxygen species production, delivery, and use in Arabidopsis root hairs.

ABSTRACT: Fluorescent selective probes for reactive oxygen species (ROS) detection in living cells are versatile tools for the documentation of ROS production in plant developmental or stress reactions. We employed high-resolution live-cell imaging and semiquantitative analysis of Arabidopsis (Arabidopsis thaliana) stained with CM-H2DCFDA, CellROX Deep Red, and Amplex Red for functional characterization of the spatiotemporal mode of ROS production, delivery, and utilization during root hair formation. Cell viability marker fluorescein diacetate served as a positive control for dye loading and undisturbed root hair tip growth after staining. Using a colocalization analysis with subcellular molecular markers and two root hair mutants with similar phenotypes of nonelongating root hairs, but with contra

SUBMITTER: Kubenova L 

PROVIDER: S-EPMC10663114 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature