GABA_B receptor outward currents are transiently disclosed by the convulsant 4-aminopyridine in vitro.

ABSTRACT: The K⁺ channel blocker 4-aminopyridine (4AP) has been extensively used to investigate the mechanisms underlying neuronal network synchronization in both in vitro and in vivo animal models of focal epilepsy. 4AP-induced effects are paralleled by an increase in both excitatory and inhibitory neurotransmitter release, but the mechanisms of action of 4AP on neuronal networks remain unclear. By employing simultaneous whole-cell patch clamp and field potential recordings from hippocampal CA3/4 pyramidal layer of acute brain slices obtained from mice (n = 30), we found that the appearance of epileptiform discharges induced by 4AP (100 μM) is consistently preceded by the transient recurrence of presumptive GABA_B outward currents, which are not mirrored by any fi

SUBMITTER: Cattani A 

PROVIDER: S-EPMC10663127 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature