3'-tRF-Cys^GCA overexpression in HEK-293 cells alters the global expression profile and modulates cellular processes and pathways.

ABSTRACT: tRNA fragments (tRFs) are small non-coding RNAs generated through specific cleavage of tRNAs and involved in various biological processes. Among the different types of tRFs, the 3'-tRFs have attracted scientific interest due to their regulatory role in gene expression. In this study, we investigated the role of 3'-tRF-Cys^GCA, a tRF deriving from cleavage in the T-loop of tRNA^CysGCA, in the regulation of gene expression in HEK-293 cells. Previous studies have shown that 3'-tRF-Cys^GCA is incorporated into the RISC complex and interacts with Argonaute proteins, suggesting its involvement in the regulation of gene expression. However, the general role and effect of the deregulation of 3'-tRF-Cys^GCA levels in human cells have not been investigated so

SUBMITTER: Karousi P 

PROVIDER: S-EPMC10663186 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature