Phylogenetic classification of natural product biosynthetic gene clusters based on regulatory mechanisms.

ABSTRACT: The natural products (NPs) biosynthetic gene clusters (BGCs) represent the adapting biochemical toolkit for microorganisms to thrive different microenvironments. Despite their high diversity, particularly at the genomic level, detecting them in a shake-flask is challenging and remains the primary obstacle limiting our access to valuable chemicals. Studying the molecular mechanisms that regulate BGC expression is crucial to design of artificial conditions that derive on their expression. Here, we propose a phylogenetic analysis of regulatory elements linked to biosynthesis gene clusters, to classify BGCs to regulatory mechanisms based on protein domain information. We utilized Hidden Markov Models from the Pfam database to retrieve regulatory elements, such as histidine kinases and transcri

SUBMITTER: Rodriguez-Sanchez AC 

PROVIDER: S-EPMC10663231 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature