Mining the Utricularia gibba genome for insulator-like elements for genetic engineering.

ABSTRACT:

Introduction

Gene expression is often controlled via cis-regulatory elements (CREs) that modulate the production of transcripts. For multi-gene genetic engineering and synthetic biology, precise control of transcription is crucial, both to insulate the transgenes from unwanted native regulation and to prevent readthrough or cross-regulation of transgenes within a multi-gene cassette. To prevent this activity, insulator-like elements, more properly referred to as transcriptional blockers, could be inserted to separate the transgenes so that they are independently regulated. However, only a few validated insulator-like elements are available for plants, and they tend to be larger than ideal.

Methods

To identify additional potential insulator-like sequences, we conducted a geno

SUBMITTER: Laspisa D 

PROVIDER: S-EPMC10663240 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature