Enhancer-driven 3D chromatin domain folding modulates transcription in human mammary tumor cells.

ABSTRACT: The genome is organized in functional compartments and structural domains at the sub-megabase scale. How within these domains interactions between numerous cis-acting enhancers and promoters regulate transcription remains an open question. Here, we determined chromatin folding and composition over several hundred kb around estrogen-responsive genes in human breast cancer cell lines after hormone stimulation. Modeling of 5C data at 1.8 kb resolution was combined with quantitative 3D analysis of multicolor FISH measurements at 100 nm resolution and integrated with ChIP-seq data on transcription factor binding and histone modifications. We found that rapid estradiol induction of the progesterone gene expression occurs in the context of preexisting, cell type-specific chromosomal architectures

SUBMITTER: Kocanova S 

PROVIDER: S-EPMC10663337 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature