Chromatin priming elements direct tissue-specific gene activity before hematopoietic specification.

ABSTRACT: Tissue-specific gene regulation during development involves the interplay between transcription factors and epigenetic regulators binding to enhancer and promoter elements. The pattern of active enhancers defines the cellular differentiation state. However, developmental gene activation involves a previous step called chromatin priming which is not fully understood. We recently developed a genome-wide functional assay that allowed us to functionally identify enhancer elements integrated in chromatin regulating five stages spanning the in vitro differentiation of embryonic stem cells to blood. We also measured global chromatin accessibility, histone modifications, and transcription factor binding. The integration of these data identified and characterised cis-regulatory elements which becom

SUBMITTER: Maytum A 

PROVIDER: S-EPMC10663361 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature